Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak qudratullah kalwar 1,2, min chu 1, anum ali ahmad 1,3, xuezhi ding 1, xiaoyun wu 1, pengjia bao 1 and ping yan 1. Statistical analysis general linear model and repeated measures. In the present study, we performed a comparative and more accurate morphometric and functional investigation of the testis in donkeys and mules. Morphometric evaluation of seminiferous tubules in aged. This is also true for primary spermatocytes of various. Due to the smaller testis size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was significantly smaller than in donkeys. In this investigation, testes of 20 donkeys ranging from birth to maturity were studied. Pdf morphometric evaluation of seminiferous tubules in aged. Tissue from perfusionfixed testes was embedded in eponaraldite. Bremner, in williams textbook of endocrinology thirteenth edition, 2016. Spermatogenic cells line the layer of sertoli cells, and they mature into fullfledged sperm through a complex process called meiosis. Effects of vitamin e on morphometric parameters of testes of albino. Male reproductive system questions and study guide. Multinucleated giant cells in seminiferous tubules are a specific form of degenerating germ cell, in contrast to nonspecific germ cell degeneration see testis, germ cell degeneration.
Morphometric studies on rat seminiferous tubules tungyang wing and a. The volume of the seminiferous epithelium per unit length of the tubule. A morphological, morphometric and ultrastructural study al moatasem bellah m. Previous studies in rats showed a relationship between testosterone levels and the morphology of seminiferous tubules. Pdf morphometric evaluation of seminiferous tubule and. Wild boars sus scrofa scrof a seminiferous tubules morphometry brazilian archives of biology and technology 741 was estimated using a 400 points grid. Morphometric studies on rat seminiferous tubules wing.
Tripathi uk, chhillar s, kumaresan a, aslam mkm, rajak sk, nayak s, manimaran a, mohanty tk, yadav s 2015 morphometric evaluation of seminiferous tubule and proportionate numerical analysis of sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls, veterinary world 85. Histopathology and morphometry of brain and seminiferous. Any of the tubercles of the testes which produce spermatozoa explanation of seminiferous tubule. A significant constriction of the seminiferous tubule accompanies sper miation.
Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak article pdf available in. The seminiferous tubules were opened into striaghted tubules which. Located in the testicles, the seminiferous tubules are tiny tubes in which spermatozoa or sperm cells are formed. Some morphometric and histological description of the. Six days after the last injection, all mice were sacrificed and the testes were excised and processed for light microscope observation. Kent christensen department of anatomy and cell biology, the university of michigan medical school, ann arbor, michigan 48109 abstract the goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of spraguedawley rats, including. The long 70 days and complex process within the seminiferous tubules of the testes, in which an elaborate succession of distinct cell types are generated by mitotic and meiotic divisions. The volume percentage of the tubular compartment seminiferous tubules was about 12. Seminiferous tubules and exploration of luteinizing. The negative correlation between the thickness of the lamina propria and johnsens score count in individual seminiferous tubules was observed and the tubules with thin lamina propria indicated almost complete spermatogenesis. Seminiferous definition is producing or bearing seed or semen. Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak by qudratullah kalwar 1,2, min chu 1, anum ali ahmad 1,3, xuezhi ding 1, xiaoyun wu 1, pengjia bao 1 and ping yan 1.
The zeiss mop3 instrument was used on selected electron microscope photographs to measure a number of morphometric parameters. Seminiferous tubules contain epithelium consisting of sertoli cells that envelop and support germ cells undergoing progressive differentiation and development into mature spermatozoa. Seminiferous tubules may constitute up to 90 percent of the testis. Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls. In melatonintreated mice, the seminiferous tubules had a wide lumen lined by low height germinal epithelium and thick interstitial connective tissue fig 2. Study of spermatogenesis and thickening of lamina propria. Tacrolimus fk506 is an immunosuppressant that binds to a specific immunophilin, resulting in the suppression of the cellular immune response during transplant rejection. Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of sertoli and spermatogenic cells indicate differences. Morphometric evaluation of seminiferous tubule and.
Wild boars sus scrofa scrof a seminiferous tubules. The walls of the seminiferous tubules contain two kinds of cells spermatogenic cells, the stem cells of sperm production, and sertoli cells, which have several functions in supporting spermatogenesis. Seminiferous tubules definition of seminiferous tubules. Seminiferous tubule an overview sciencedirect topics. As a result of internalization of the tracer by sertoli cells, label was found within 1266% of the secondary lysosomes, depending on the stage of the cycle of the seminiferous epithelium. In the control group, the testes consisted of a number of seminiferous tubules lined by the germinal epithelium and separated by interstitial connective tissue fig 1. However, probably due to the fact that the tubular diameter in domestic cats is not high and that almost 90% of seminiferous tubules are found in testis parenchyma, approximately 20 m of tubules per gram are observed in this species. Once released into the lumen, mature spermatozoa are transported.
Seminiferous tubule article about seminiferous tubule by the free dictionary. One of two or three twisted, curved tubules in each lobule of the testis in which spermatozoa develop. Some of the seminiferous tubules showed distorted germinal epithelium with its vacuolization and stopping the development of germinal cells. Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak.
The expected protective role of celery oil on induced. The area of the seminiferous tubule ranged from 15400 m 5 to 97096 m2 with a mean of 43309. Cyclic endocytic activity and kinetics of lysosomes in. The postnatal morphologic and morphometric characteristics of donkeys seminiferous epithelium during the postnatal period were studied. Dawley rats, including changes seen at various stages of. They are lined with sertoli cells, specialized cells that serve as a sort of nursery for growing sperm cells. From each section, seminiferous tubule diameter and area essentially from circular tubular cross sections was determined using precalibrated measuring eyepiece.
The diameter and length of seminiferous tubules and diameter of lumen were measured from twenty five 25 randomly selected tubules in each prepared tissue sample with a total of seventy five 75 tubules per replication. Changes occur in the diameter of the seminiferous tubule, as well as in the vol ume of the seminiferous epithelium and tubule lumen, from stage to stage during the cycle. Learn more about the word seminiferous tubules, its origin, alternative forms, and usage from wiktionary. The described testicular malformations in immunocastrated animals caused their lower weight, and greater. The presence of giant cells can be focal or diffuse and. The spermatozoa develop from cells in the walls of these tubules. To study morphological changes of the seminiferous tubules and fluctuations on the scrotal circumference at two different. Morphometry of testis and seminiferous tubules of the adult crabeating fox cerdocyon thous, linnaeus, 1766. In kf bulls, the diameter of seminifer ous tubules ranged from 14747 m2 to 57172 m2.
Pdf stereological and morphometric analysis of collagen. Morphometric evaluation of seminiferous tubules from each section, seminiferous tubule diameter and area essentially from circular tubular cross sections was determined using precalibrated measuring eyepiece. The goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of spraguedawley rats, including changes seen at various stages of the cycle of the seminiferous epithelium. Morphometric evaluation of seminiferous tubules in aged mice testes after melatonin administration. The quantitative histology of the testicles, e ciency of leydig and sertoli cells, and general morphometry of the seminiferous tubule may be related to the quantity of spermatozoa in the ejaculate, as well as reproductive e ciency 5,6. Morphometric studies on rat seminiferous tubules deep blue.
The seminiferous tubules of the testis are the convoluted tubules where sperm are produced. Seminiferous tubule definition is any of the coiled threadlike tubules that make up the bulk of the testis and are lined with a layer of epithelial cells from which the spermatozoa are produced. It is suggested that the thin lamina propria may be one of the good indicators of complete spermatogenesis. Seminiferous tubule definition of seminiferous tubule by. Except for some alterations in the spermatozoa, testicular morphological alterations have not been described in rats treated with tacrolimus. The seminiferous tubules and the intertubular tissue were computed. Morphometric evaluation of spermatogenic cells and. Pdf morphometric evaluation of seminiferous tubules in. Morphometric evaluation of spermatogenic cells and seminiferous. Stereological and morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human.
Due to the smaller testis size, lower seminif erous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was significantly. Histological and morphometric examination of the testes. Morphometry of testis and seminiferous tubules of the adult crab. Other articles where seminiferous tubule is discussed. Vacuoles are present in affected seminiferous tubules. In the present study, we purpose to evaluate if the treatment with.
Morphological and morphometric study of the development. Figure 1 testis, seminiferous tubule necrosis in a male swiss webster mouse from a chronic study. The number of sertoli cells is constant per unit length of the seminiferous tubule at all stages of the cycle. Morphometric study of the testes of the nigerian local breed of chicken f, animal research international 2010 72. Pdf morphometric evaluation of spermatogenic cells and. The goal of this morphometric study was to obtain quantitative information on the seminiferous tubules of sprague. Comparative testis morphometry and seminiferous epithelium.
Seminiferous definition of seminiferous by merriamwebster. The epithelium of the tubule consists of a type of sustentacular cells known as sertoli cells, which are tall, columnar type cells that line the tubule. Seminiferous tubule article about seminiferous tubule by. Morphometric study a morphometric study for each chosen seminiferous tubule that included external seminiferous tubular diameter std and seminiferous epithelial height seh were measured by olysia bio software. Morphometric studies on rat seminiferous tubules wing 1982. The point counting method was applied to estimate the volume density and volume of the seminiferous tubules and interstitial tissue.
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